By Ronald Blythe
Woven from the phrases of the population of a small Suffolk village within the 1960s, Akenfield is a masterpiece of twentieth-century English literature, a scrupulously saw and deeply affecting portrait of a spot and folks and a now vanished lifestyle. Ronald Blythe's marvelous booklet increases enduring questions on the kinfolk among reminiscence and modernity, nature and human nature, silence and speech.
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Diol is capable of directly modulating oxytocin gene expression in the rat paraventricular nucleus, while no signiﬁcant effect was seen in the supraoptic nucleus. It is interesting to note that a single bolus of estradiol decreased the level of oxytocin mRNA. , 1999). While the reason for these differences is unknown, it is noteworthy that the treatment paradigm used in our studies (single injection, rapid uptake) is clearly different than the methods used by most investigators (single or mul- tiple injections; slow, sustained delivery).
1996). , 1998). g. g. g. paraventricular and supraoptic nuclei, cerebellum). In the rat diencephalon, intense hybridization signal was seen in almost every neuron of the supraoptic nucleus (Fig. 1B), extending throughout the length of the hypothalamus and in the retrochiasmatic supraoptic nucleus. In addition, numerous ER-β-containing neurons were concentrated in the paraventricular nucleus (Fig. 1A). The majority of the intensely labeled cells were located in the magnocellular subdivisions, with the posterior subnucleus having the highest number of labeled cells (Fig.
In an attempt to demonstrate that ER-β mRNA was translated into a biologically active protein, a series of in vivo binding studies were conducted in rats with 125 I-estrogen. These data revealed the presence of nuclear estrogen binding sites in neurons of the magnocellular system indicating that ER-β mRNA was translated into protein. Concurrent studies in mice found that the distribution of ER-β mRNA and 125 I-estrogen binding was similar to rats, although there were some notable differences. For example, ER-β mRNA and binding were not detected in the mouse supraoptic nucleus and although ER-β was the principle ER in the paraventricular nucleus, ER-α was also present.
Akenfield: Portrait of an English Village by Ronald Blythe