By Louis Sokoloff (auth.), B. W. Agranoff, M. H. Aprison (eds.)
This sequence has been directed at offering scientists owning significant bio chemical heritage with really good studies of neurobiological curiosity. a few have handled accomplished our bodies of analysis, whereas others include vast reviews of analysis in development, judged to be of present curiosity to the lively researcher. we've got chosen famous scientists and allowed them freedom to mirror and speculate within the box within which they've got completed promenade inence. We be aware with disappointment the passing of Dr. 10rdi FoIch-Pi, who served as an advisory editor while the sequence was once initiated. He performed a significant position within the improvement of neurochemistry, in addition to the production societies and journals. he'll be remembered fondly by means of all these whose lives he touched. The editors recognize the cooperation of the Upjohn corporation within the practise of the colour plate incorporated during this quantity. We additionally recognize the skillful editorial tips of Dr. Kenneth C. Leskawa. we're happy to honor the retirement of Dr. E. Martin Gal, a former advisory editor of Advances, with the inclusion of a bankruptcy via him during this volume.
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There was no trace of [14C]-DG-6-P in any of the samples. Despite the fact that there was clearly a net loss of 14C from the brain to the blood during this period, all of the loss could be accounted for as [14C]-DG. These results not only confirmed that there is bidirectional transport of free [14C]-DG between blood and brain but also demonstrated that there is no direct loss of [14C]-DG-6-P from the cerebral tissues to the blood. It was possible that some of the [14C]-DG lost from the brain tissues to the blood was derived from the hydrolysis of [14C]-DG-6-P.
Hawkins and Miller (1978) attempted to evaluate this activity as well. They applied the [14C]-DG method to a series of rats but killed them at various times after the intravenous pulse of [14C]-DG and measured the [14C]-DG-6-P concentrations in brain as a function of time by direct chemical analyses. , the numerator of the operational equation) predicted by the equation, which assumes no loss of [14C]-DG-6-P owing to phosphatase activity. Their computed curve was 2- to 3-fold higher than their measured concentrations (Figure 6), and they interpreted their results as proof that there was significant loss of [14C]-DG-6-P during the experimental period.
Sokoloff, unpublished data); dog, Duffy el al. (1979) (From Sokoloff, 1979). 9). (k! ) in Table 1, the exponential factors have declined through at least ten half-Iiv~s. The time courses of the concentrations of [14C]-DG and [14C]-DG-6-P in arterial plasma and/or representative gray and white matter following an intravenous pulse of [14C]-DG are illustrated in Figure 4. As the plasma concentration falls from its peak following the pulse, the tissue concentrations of
Advances in Neurochemistry by Louis Sokoloff (auth.), B. W. Agranoff, M. H. Aprison (eds.)