By Gerd Plewig Prof. Dr., Albert M. Kligman M.D., Ph.D. (auth.)
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J Invest Dermatol 54: 487--495 Perisho K, Wertz PW, Madison KC, Stewart ME, Downing DT (1988) Fatty acids of acylceramides from comedones and from the skin surface of acne patients and control subjects. J Invest Dermatol 90:350-353 Stewart ME (1992) Sebaceous gland lipids. Semin Dermatol 11: 100-105 Dilution of the epidermal lipids of the follicular epithelium by sebaceous lipids. FFA free fatty acids, WE wax esters, TG triglycerides, sa squalene Not only ceramides but also other epidermal lipids such as free sterols are reduced in acne comedones.
Electron microscopy, x 16600) Below: Courtesy of Professor Helmut H. Wolff, Lubeck, FRG 48 Comparative Ultrastructure of the Acroinfundibulum and the Infrainfundibulum Above The acroinfundibulum. The keratinizing epithelium is on the left with prominent keratohyalin granules. Rows of dense corneocytes on the right are compacted into a horny layer. This corresponds to the interfollicular portion of the epidermis. The acroinfundibulum is an extension of the epidermis into the terminal segment of a sebaceous follicle.
Sections of this extent are not easily obtained. This in the midportion of a normal sebaceous follicle from the back of a young man. The follicular epithelium is in the upper right and lower left corners. The hair with its cuticule is in the center. About 15 layers of corneocytes line the epithelium; the outermost layers detach and desquamate in an amalgam of sebum. One cannot see bacteria in this section, although present elsewhere in the follicle. (Electron microscopy, x 4000) 20 Sampling of Follicles by the Cyanoacrylate Technique Cyanoacrylate, also known as crazy glue, is a quick-setting polymer which rips off the outermost layers of the stratum corneum, to which are attached vellus hairs and the horny casts within the upper regions of sebaceous follicles.
ACNE and ROSACEA by Gerd Plewig Prof. Dr., Albert M. Kligman M.D., Ph.D. (auth.)